Amateur soccer players are exposed to significant physical efforts, which is why regular medical examinations are crucial for injury prevention and health preservation. The aim of this research was to examine the knowledge, attitudes and practice of amateur soccer players in Osijek-Baranja County regarding the implementation of physical examinations and to determine the need for additional education. The research was conducted on a sample of 77 amateur soccer players. Data were collected using an anonymous survey questionnaire that included sociodemographic characteristics, knowledge, attitudes and practices about health examinations. The results show that 49% of respondents undergo a physical examination once a year, 35% twice a year, while 16% do not go for examinations at all. A significant number of respondents (20%) avoid examinations when possible. Also, respondents from rural areas recognize the importance of medical examinations less than those from urban areas. A correlation was established between lower knowledge about health examinations of athletes and agreement with the statement that examinations are useless and only necessary for soccer players with existing health problems, while respondents with greater knowledge are more likely to recognize that examinations are extremely important in amateur and professional sports and that regular preventive examinations can save lives, which speaks in favor of additional educational content and the high potential of such content to improve adherence and understanding of the importance of these examinations.